氨基糖苷类药物是一类广谱抗菌药物,主要从链霉菌属和小单孢菌属中分离获得,该类药物能够治疗多种感染性疾病以及农作物腐烂病等,在生物医疗、农业等方面具有重要作用.为发现和挖掘新的氨基糖苷类天然产物提供种质资源、丰富氨基糖苷类化合物,本研究从沙漠土壤中分离获得链霉菌属,通过"基因-活性-化学"相结合的筛选手段从132株链霉菌中筛选出两株具有产氨基糖苷类化合物潜力的菌株:TRM71140和TRM94015.通过质谱法检测,从TRM94015发酵产物中检测到大观霉素信号,该研究结果丰富了氨基糖苷类化合物潜力菌株.
Aminoglycosides are a class of broad-spectrum antibacterial drugs,which are mainly isolated from Streptomyces and Mi-cromonospora;this type of drug can treat a variety of infectious diseases and crop rot,etc,which plays an important role in biomedi-cine,agricultural and other fields.In order to provide germplasm resources and enrich aminoglycoside compounds for the discovery and excavation of new aminoglycoside natural products.Through the combination of"gene-activity-chemistry"screening method,TRM71140 and TRM94015 with aminoglycoside production potential were selected from 132 Streptomyces.The spectinomycin signal was detected from the TRM94015 fermentation product by mass spectrometry,which enriched the potential strains of aminoglycoside compounds.
张嘉馨;骆新荣;刘占文;高佳敏;罗晓霞;张利莉
塔里木大学生命科学与技术学院,新疆阿拉尔 843300||塔里木盆地生物资源保护利用兵团重点实验室-省部共建国家重点实验室培育基地,新疆阿拉尔 843300
生物学
沙漠链霉菌;次级代谢产物挖掘;氨基糖苷类药物
Streptomyces deserticus;secondary metabolite mining;aminoglycosides
《塔里木大学学报》 2024 (001)
13-21 / 9
国家自然科学基金-新疆联合基金重点项目(U1703236)
10.3969/j.issn.1009-0568.2024.01.002
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