慢加急性肝衰竭合并细菌感染患者的临床特征及早期预警指标筛选OACSTPCD

Objective To investigate the clinical features of patients with acute-on-chronic liver failure(ACLF)and bacterial infection and early warning indicators associated with multidrug-resistant infections.Methods A retrospective analysis was performed for 130 patients with ACLF and bacterial infection who attended The Second Affiliated Hospital of Air Force Medical University from January 1,2010 to December 31,2021,and according to the drug susceptibility results,the patients were divided into multidrug-resistant(MDR)bacterial infection group with 80 patients and non-MDR bacterial infection group with 50 patients.General information and laboratory examination results were compared between the two groups to screen for the early warning indicators associated with MDR bacterial infection.The Student's t-test was used for comparison of normally distributed continuous data with homogeneity of variance between two groups,and the Mann-Whitney U test was used for comparison of non-normally distributed continuous data or continuous data with heterogeneity of variance between two groups;the chi-square test or the Fisher's exact test was used for comparison of categorical data between two groups.The binary logistic regression analysis and the receiver operating characteristic(ROC)curve were used to assess the predictive value of early warning indicators.Results Among the 130 patients with ACLF and bacterial infection,sputum(27.7%)was the most common specimen for detection,followed by blood(24.6%),urine(18.5%),and ascites(17.7%).Bacterial infections were dominated by Gram-negative bacteria(58.5%).Of all bacteria,Escherichia coli(18.5%),Klebsiella pneumoniae(14.6%),and Enterococcus faecium(13.8%)were the most common pathogens.Gram-positive bacteria had a high resistance rate to the antibacterial drugs such as erythromycin(72.2%),penicillin(57.4%),ampicillin(55.6%),and ciprofloxacin(53.7%),while Gram-negative bacteria had a high resistance rate to the antibacterial drugs such as ampicillin(73.3%),cefazolin(50.0%),and cefepime(47.4%).The patients with ACLF and bacterial infection had a relatively high rate of MDR bacterial infection(61.5%).Comparison of clinical data between the two groups showed that compared with the patients with non-MDR bacterial infection,the patients with MDR bacterial infection had significantly higher levels of alanine aminotransferase(Z=2.089,P=0.037),aspartate aminotransferase(Z=2.063,P=0.039),white blood cell count(Z=2.207,P=0.027),and monocyte count(Z=4.413,P<0.001).The binary logistic regression analysis showed that monocyte count was an independent risk factor for MDR bacterial infection(odds ratio=7.120,95%confidence interval[CI]:2.478—20.456,P<0.001)and had an area under the ROC curve of 0.686(95%CI:0.597—0.776)in predicting ACLF with MDR bacterial infection(P<0.001),with the optimal cut-off value of 0.50×109/L,a sensitivity of 0.725,and a specificity of 0.400.Conclusion ACLF combined with bacterial infections is mainly caused by Gram-negative bacteria,with the common pathogens of Escherichia coli and Klebsiella pneumoniae and a relatively high MDR rate in clinical practice.An increase in monocyte count can be used as an early warning indicator to distinguish MDR bacterial infection from non-MDR bacterial infection.