湘黄精中高异黄酮类化学成分研究OACSTPCD

Objective To investigate the chemical components of dried rhizomes of Polygonatum hunanense H.H.Liu&B.Z.Wang,a plant belonging to the genus Polygonatum.Methods The methods such as Silica gel,Sephadex LH-20 gel,and semi-preparative HPLC were used for the isolation and purification of the ethyl acetate part of P.hunanens-e,and then the structures of the compounds obtained were identified based on spectral data and physicochemical properties.Results A total of 13 compounds were isolated from the 95%ethanol extract of the rhizome of P.hunanense,which were identified as 5,7-di-hydroxy-6-methyl-3-(4-methoxybenzyl)-chroman-4-one(1),5,7-dihydroxy-3-(2-hydroxy-4-methoxybenzyl)-chroman-4-one(2),ophiopogonanone G(3),3-(4′-hydroxy-benzyl)-5,7-dihydroxy-6-methyl-chroman-4-one(4),5,7-dihydroxy-6,8-dimethyl-3-(2′-hydroxy-4′-methoxybenzyl-)chroman-4-one(5),3-(2′,4′-dihydroxybenzyl)-5,7-dihydroxy-chroman-4-one(6),polygona-tone H(7),4′,5,7-trihydroxy-6,8-dimethylhomoisoflavanone(8),3-(4′-hydroxybenzylidene)-5,7-dihydroxychroman-4-one(9),daucosterol(10),linoleic acid(11),β-sitosterol(12),and methyl hexadecanoate(13).Conclusion Compound 13 is isola-ted from Polygonatum for the first time,and compounds 1-13 are isolated from P.hunanense for the first time.