昆虫视觉电生理技术是研究昆虫感光细胞和视觉神经元电学特性的重要技术,包括视网膜电位技术(ERG)和微电极细胞内记录技术(MIR).ERG技术记录的是昆虫的视网膜对不同光刺激产生的电压或电流变化,这种反应发生在细胞外.MIR则是记录昆虫的单个感光细胞或视觉神经元对不同光刺激产生的电压或电流变化,这种反应发生在细胞内.昆虫电生理技术有助于探究昆虫视觉对光的电生理响应机制和明确昆虫敏感光谱和光感受器类型.本文介绍了 ERG和MIR的基本结构及原理,总结了近20年来两种技术在昆虫感光电生理方面的应用,可为阐明昆虫对光的感受机制以及利用昆虫的趋光性防治害虫提供参考.
Insect visual electrophysiological techniques are important to study the electrical characteristics of photo-receptor cells and visual neurons in insects,including electroretinography(ERG)and microelectrode intracellular recording(MIR).ERG records the changes of voltage or electric current in the retina of insects in response to dif-ferent light stimuli,which occurs outside the cell.MIR records the changes in individual photoreceptor cells or visual neurons of an insect exposed to different lights,which occurs inside the cell.Insect visual electrophysiological tech-niques can explore the mechanism of electrophysiological response of insects'vision to light and reveal their sensi-tive light spectra and photoreceptor types.This review introduced the basic structure and the principle of ERG and MIR,and summarized their applications in insect researches in the past 20 years,which would provide references for elucidating the mechanism of light perception in insects and the use of insect phototropism to control pests.
刘航;魏成梅;冯太阳;董文霞;肖春
云南生物资源保护与利用国家重点实验室,昆明 650201||云南农业大学植物保护学院,昆明 650201
视网膜电位;微电极细胞内记录;敏感光谱;光感受器
electroretinography;microelectrode intracellular recording;sensitive light spectra;photoreceptor
《应用生态学报》 2024 (003)
858-866 / 9
本文由云南省科技厅重大专项(202102AE090006)资助.
10.13287/j.1001-9332.202403.032
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