羟基磷灰石(HAP)作为一种常见的骨修复材料,在治疗感染性骨缺损时仍面临着细菌感染的风险,有限的骨诱导能力也阻碍了其进一步应用.本研究采用共沉淀法制备了一种锰掺杂羟基磷灰石纳米棒(MnHAP),该纳米材料兼具优良的细胞生物相容性,高抗菌效率和骨诱导能力.抗菌实验表明,MnHAP10(n(Mn)/n(Ca+Mn)=10%))对大肠杆菌和金黄色葡萄球菌的抑制率可分别达到77.85%和75.92%,并且在808 nm近红外光照射下,对大肠杆菌的抗菌效率得到了进一步增强(97.63%).细胞增殖以及相关成骨基因实验表明,MnHAP有利于成骨细胞的增殖分化,提高了对蛋白质的吸附能力和成骨活性,促进了相关成骨基因的表达,且具有良好的生物相容性.MnHAP 纳米棒有望为感染性骨缺损治疗提供一种新的思路.
Hydroxyapatite(HAP),as a common bone repair material,still faces the risk of bacterial infection in the treatment of infectious bone defects,whose limited osteogenic properties also hinders its further application.This study used a coprecipitation method to prepare the manganese doped hydroxyapatite nanorod(MnHAP),which exhibited excellent cell biocompatibility,high antibacterial efficiency and osteogenic properties.Antibacterial experiments showed that the inhibition rates of MnHAP10(n(Mn)/n(Ca+Mn)=10%))against Escherichia coli and Staphylococcus aureus can reach 77.85%and 75.92%,respectively.Moreover,the antibacterial efficiency of MnHAP10 against Escherichia coli can be further enhanced(97.63%)under 808 nm near-infrared light irradiation.Cell proliferation and related osteogenic gene experiments display that MnHAP is beneficial for the proliferation and differentiation of osteoblasts,which improves protein adsorption capacity,stimulates osteogenic activity,and promotes the expression of related osteogenic genes,demonstrating its good biocompatibility.Therefore,MnHAP nanorods are expected to provide a new approach in the treatment of infectious bone defects.
李承瑜;丁自友;韩颖超
武汉理工大学 材料复合新技术国家重点实验室,武汉 430070
化学工程
羟基磷灰石;锰;抗菌;感染性骨缺损;光热
hydroxyapatite;manganese;antibacterial;infectious bone defect;photothermal
《无机材料学报》 2024 (003)
313-320 / 8
国家自然科学基金(51672206)National Natural Science Foundation of China(51672206)
10.15541/jim20230438
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