高温胁迫对大白菜类胡萝卜素的影响OACSTPCD

In order to investigate the response mechanism of Chinese cabbage leaf blades under high temperature stress,Chinese cabbage 245,Gailiang Qingza 3 and Ziyi with different leaf colors and stress resistance were used as experimental materials,and their seedlings were subjected to heat stress at 40℃/30℃and 60%relative humidity.The effects of stress on the growth and physiological characteristics of Chinese cabbage seedlings were researched.the results showed that the carotenoids in leaf blades of three Chinese cabbage varieties showed a trend of first decrease and then increase under heat treatment.With the increase of heat stress duration,the carotenoid content of Chinese cabbage 245 was lowest,with 123.51 μg/g at the 6th day,and then peaked to 148.64 μg/g at the 10th day.Gailiang Qingza 3 and Ziyi showed different degrees of fluctuation,and Gailiang Qingza 3 was more sensitive to heat treatment,reaching the lowest value of 97.54 μg/g at the 4th day.The carotenoid content of Ziyi showed little difference and reached the lowest level of 124.50 μg/g at the 8th day under heat treatment.Real-time fluorescence quantitative PCR results showed that BraA01g019700.3C and BraA04g000960.3C in Chinese cabbage were significantly responsive to heat stress.The transcription levels of carotenoids related genes BraA01g019700.3C and BraA04g000960.3C in Chinese cabbage increased first and then decreased.The correlation analysis results showed that the transcription abundance of BraA05g001160.3C(P＜0.01,r=0.000)and BraA01g019200.3C(P＜0.05,r=0.040)was significantly correlated with carotenoid content.