建立高效液相色谱法测定婴幼儿配方乳粉中3 种醛类(丙二醛、丙烯醛、4-羟基己烯醛)含量的分析方法,研究贮藏温度对婴幼儿配方乳粉中3 种醛类含量的影响.考察线性关系、精密度、回收率、检出限及定量限等指标,外标法定量.结果表明:该方法在0～40 μg范围内线性良好(r＞0.999),3 种醛类检出限分别为0.3、0.4、0.3 mg/kg,定量限分别为0.8、1.0、0.8 mg/kg,平均加标回收率为88.29%～96.87%,相对标准偏差均小于10%;当乳粉贮藏环境温度高于40℃,随着温度升高,3 种醛类含量均呈上升趋势,以丙二醛与丙烯醛上升显著.该研究方法具有快速、准确、重复性好等特点,能满足婴幼儿配方乳粉中3种醛类的测定.
A high performance liquid chromatography(HPLC)method was established to determine the contents of three aldehydes in infant formula milk powder,and the effect of storage temperature on the content of three aldehydes in infant formula milk powder was studied.The analytical figures of merit including linearity,accuracy,recovery,detection limit and quantitation limit were determined.The external standard method was used to quantify the analytes.The results indicated that this method exhibited good linearity in the range between 0 and 40 μg with a correlation coefficient(R2)greater than 0.999,and the detection limits for malondialdehyde,acrolein,and 4-hydroxyhexenal were 0.3,0.4,and 0.3 mg/kg,respectively.The quantitation limits were 0.8,1.0 and 0.8 mg/kg,respectively.The average recoveries for spiked samples were in the range of 88.29%-96.87%,with relative standard deviation(RSD)less than 10%.With increasing storage temperature above 40℃,the contents of the three aldehydes showed an upward trend,and a significant increase was observed in the contents of malondialdehyde and acrolein.This method has the characteristics of rapidity,high accuracy,and good reproducibility,which is suitable for the determination of the three aldehydes in infant formula milk powder.
邓晓庆;郑利军;唐万里;文欣;唐文杰;陈同强
湖南振华检测技术有限公司,湖南 长沙 410000中大智能科技股份有限公司,湖南 长沙 410000湖南省产商品质量检验研究院,湖南 长沙 410007
轻工业
高效液相色谱法;婴幼儿配方乳粉;醛类
high performance liquid chromatography;infant formula milk powder;aldehydes
《乳业科学与技术》 2023 (006)
25-28 / 4
10.7506/rykxyjs1671-5187-20231205-059
评论