茉莉酸甲酯调控牛大力有效成分积累及相关基因表达OACSTPCD

Objective:To study the effect of methyl jasmonate(MeJA)on the content of active components in Callerya speciosa,and explore the transcriptional regulation mode of MeJA on key enzyme genes in isoflavonoid biosynthesis and jasmonate(JA)signaling pathway.Methods:Using the three-month seedlings of Callerya speciosa as materials,the effect of MeJA on the contents of active compo-nents was analyzed by HPLC,transcriptome sequencing was performed by Illumina HiSeq platform,and the expressions of related genes were verified by qRT-PCR.Results:Exogenous MeJA increased the contents of index components in Callerya speciose,including form-ononetin and maackiain.Based on the transcriptome data of Callerya speciosa,a total of 20 727 differentially expressed genes(CDEGs)were found,of which 9 091 were up-regulated.KEGG enrichment analysis showed that DEGs were significantly enriched in plant hor-mone signal transduction,starch and sucrose metabolism,isoflavonoid biosynthesis etc.The qRT-PCR analysis confirmed the validity of the transcriptome data,indicating that the transcriptome data were reliable and accurate.Exogenous MeJA activated some key enzyme genes in isoflavonoid biosynthesis and JA signal pathway in Callerya speciosa.Among them,the flavonoid biosynthetic genes CHS,HIDs,I3'Hs,VR,PTR,CYP81E7,7IOMT and PTS and the genes involved in the JA signaling pathway,such as LOXs、AOSs、OPR、ACXs、JAZs and MYCs,were up-regulated.Conclusion:The contents of formononetin and maackiain are significantly increased through achieving by enhancing the expressions of LOX and MYC involved in JA signaling pathway and isoflavonoid biosynthetic genes CHS,HID-1 and VR response to exogenous MeJA in Callerya speciosa.