由禾谷镰刀菌Fusarium graminearum引起的小麦赤霉病(Fusarium head blight,FHB)是小麦、大麦、燕麦、黑麦等禾谷类作物的毁灭性病害.目前,生产上防治小麦赤霉病主要依靠化学药剂防治,多菌灵等苯并咪唑类杀菌剂使用较为广泛,其作用靶标为β微管蛋白.禾谷镰刀菌有2个β微管蛋白,通过分子对接结果发现β2微管蛋白第138位氨基酸位点可能为多菌灵结合位点.本研究对β2第138位丝氨酸位点进行突变研究,以明晰其生物学功能.结果表明Fgβ2 S138A突变后禾谷镰刀菌对多菌灵的敏感性显著增加,EC50值由0.617 mg/L降至0.290 mg/L,但不影响对噻菌灵的敏感性,EC50值为0.950 mg/L左右,并且该突变不影响禾谷镰刀菌菌丝营养生长、无性繁殖、有性生殖和致病性.本研究结果可为多菌灵对小麦赤霉病的化学防治提供理论基础,在生产上具有一定指导意义.
Fusarium head blight(FHB)caused by Fusarium graminearum is a devastating disease of wheat,barley,oats,rye,and other cereal crops.Currently,the control of FHB in production mainly depends on chemical agents,among which carbendazim and other benzimidazole fungicides are widely used.The target of these fungicides is β-tubulin.There are two β tubulins in F.graminearum.A previous study showed that the 138th amino acid of β2-tubulin might be a carbendazim binding site.In this study,a Fgβ2 Ser138 mutation was constructed to clarify its biological function.The results showed that the sensitivity of Fgβ2 S138A mutation to carbendazim was significantly increased,and the EC50 value decreased from 0.617 mg/L to about 0.290 mg/L,however,the mutations did not affect the sensitivity to thiabendazole,and the EC50 value was about 0.950 mg/L,and all of them did not affect the vegetative growth,asexual reproduction,sexual reproduction and pathogenicity of F.graminearum.These results provide a theoretical basis for the chemical control of carbendazim against FHB and have a particular guiding significance in production.
蒋凡;吕俊博;赵彦翔;张迎新;黄金光
青岛农业大学植物医学学院/山东省高校植物病虫害绿色防控工程研究中心,青岛 266109青州市农业技术推广中心,青州 262500
植物保护学
禾谷镰刀菌;小麦赤霉病;药剂敏感性;定点突变
Fusarium graminearum;Fusarium head blight;fungicide sensitivity;site-directed mutagenesis
《中国生物防治学报》 2024 (001)
206-218 / 13
国家自然科学基金(31871916);国家重点研发项目(2022YFD1400104)
10.16409/j.cnki.2095-039x.2023.02.072
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