为筛选出具有促生效果的生防细菌,本研究从河北、山东等地采集土样,分离筛选具有高产IAA能力的促生菌株,基于形态学特征、生理生化指标及多基因系统发育树分析,对潜在具有促生能力的菌株进行鉴定.同时测定促生菌株对黄瓜种子出芽及在盆栽试验、田间试验中对黄瓜幼苗的促生效果.结果表明:分离并鉴定出1株对黄瓜幼苗具有显著促生效果的细菌,滋养节杆菌Arthrobacter pascens S17.在DF+培养基中滋养节杆菌S17可大量合成IAA,其合成的IAA含量最高为128.54 μg/mL.使用滋养节杆菌S17灌根处理,对黄瓜幼苗株高的增长率达到35.99％、对黄瓜幼苗叶面积的增长率达到26.90％,黄瓜幼苗各项生理指标均显著高于对照.滋生节杆菌S17对黄瓜促生效果显著,具有一定的开发应用前景.
In order to screen out the biocontrol bacteria with growth-promoting effect,soil samples were collected from Hebei,Shandong and other places,and the growth-promoting strains with high-yield IAA ability were isolated and screened,and the potential growth-promoting strains with growth-promoting ability were identified based on morphological characteristics,physiological and biochemical indexes and multi-gene phylogenetic tree analysis,and the growth-promoting effects of growth-promoting strains on cucumber seed germination and cucumber seedlings in pot experiments and field experiments were determined.The results showed that one bacterium,Arthrobacter pascens S17,which had a significant growth-promoting effect on cucumber seedlings,was isolated and identified.Arthrobacter pascens S17 could synthesize IAA in large quantities in DF+medium,and the highest IAA content was 128.54 μg/mL.The growth rate of cucumber seedling plant height and leaf area of cucumber seedlings reached 35.99％and 26.90％by root irrigation treatment with Arthrobacter pascens S17,and the physiological indexes of cucumber seedlings were significantly higher than those of the control.Arthrobacter pascens S17 has a significant effect on cucumber growth,and has a certain development and application prospect.
曾先锋;孙钰晨;谢学文;石延霞;柴阿丽;李宝聚;李磊
中国农业科学院蔬菜花卉研究所/蔬菜生物育种全国重点实验室,北京 100081中国农业科学院蔬菜花卉研究所/蔬菜生物育种全国重点实验室,北京 100081||天津农学院园艺园林学院,天津 300384
植物保护学
滋养节杆菌;鉴定;促生;种子处理;田间试验
Arthrobacter pascens;indentification;growth-promoting;seed treatment;field trials
《中国生物防治学报》 2024 (001)
178-185 / 8
北京设施蔬菜创新团队项目(BAIC01-2023);中国农业科学院科技创新工程项目(CAAS-ASTIP-IVFCAAS);农业农村部园艺作物生物学与种质创制重点实验室项目;蔬菜生物育种全国重点实验室
10.16409/j.cnki.2095-039x.2023.02.070
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