由灰葡萄孢菌Botrytis cinerea引起的草莓灰霉病是草莓栽培中最具破坏性的病害之一,严重降低食用以及经济价值.本研究以"红颜"草莓为试验材料,测定砖红镰刀菌Fusarium lateritiumPa2对草莓灰霉病菌的抑菌活性、防治效果以及草莓病程相关基因表达水平的影响.结果表明,菌株Pa2对草莓灰霉病菌具有显著的抑菌活性,其上清液可以破坏草莓灰霉病菌菌丝细胞膜,导致细胞内容物泄露.离体接种试验结果表明菌株Pa2对草莓叶片灰霉病、草莓果实灰霉病防治效果分别为79.13％、54.29％.经菌株Pa2上清液处理24 h后草莓果实病程相关基因FaPR1、FaPR4、FaGR分别上升19.05、24.64、3.69倍.综上所述,砖红镰刀菌菌株Pa2是一种防治草莓灰霉病的新型生防菌资源.
Strawberry gray mold caused by Botrytis cinerea is one of the most destructive diseases in strawberry cultivation,which seriously reduces the edible and economic value.In this study,"Hongyan"strawberries were used as experimental materials to determine the effects of Fusarium lateritium Pa2 on the inhibitory activity and control of gray mold pathogen,as well as the effects on the pathogenesis-related genes expression levels of strawberry fruit.The results showed that strain Pa2 had significant antifungal activity against B.cinerea.The supernatant of strain Pa2 could damage the mycelial cell membrane of B.cinerea,leading to leakage of cell contents.The results of inoculation experiments indicated that the control effects of strain Pa2 on strawberry leaves gray mold and strawberry fruits gray mold were 79.13％and 54.29％,respectively.In addition,the strawberry fruits pathogenesis-related genes FaPR1,FaPR4,and FaGR increased by 19.05,24.64,and 3.69 times at 24 hours post treatment with supematant of strain Pa2,respectively.In conclusion,the F.lateritium strain Pa2 is a new biocontrol resource for controlling strawberry gray mold.
史冰柯;李晓倩;王卓妮;覃艮红;范洋洋;王丽;涂洪涛;袁洪波;侯珲
中国农业科学院郑州果树研究所,郑州 450009中国农业科学院郑州果树研究所,郑州 450009||中国农业科学院中原研究中心,新乡 453004
植物保护学
草莓灰霉病;砖红镰刀菌;病程相关基因;防治效果
strawberry gray mold;Fusarium lateritium;pathogenesis-related genes;control effect
《中国生物防治学报》 2024 (001)
137-145 / 9
国家重点研发计划(2022YFD1600702);中国农业科学院科技创新工程(CAAS-ASTIP-2016-RIP);中国农业科学院郑州果树研究所科研业务费专项(ZGS202205 and 1610192023311)
10.16409/j.cnki.2095-039x.2024.02.001
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