目的:探究金银花提取物(HFE)联合奥曲肽(Octreotide)对胰腺炎大鼠腺泡细胞损伤修复、免疫学指标及p38丝裂原活化蛋白激酶(p38MAPK)、激活转录因子2(ATF2)水平的影响.方法:将SD大鼠分为健康组、模型组、Octreotide组、HFE+Octreotide组.除健康组外均建立胰腺炎大鼠模型,健康组、模型组用1 mL/kg生理盐水腹腔注射、HFE+Octreotide组尾静脉注射HFE(120 mg/kg)和Octreotide(10 μg/kg)、Octreotide组尾静脉注入Octreotide(10 μg/kg),均1天1次,共4周.采用ELISA法检测血清免疫球蛋白IgA、IgG、IgM含量,免疫组织化学法检测胰腺组织p38MAPK、ATF2水平,H-E染色观察胰腺组织病理变化,采用比色法检测腺泡细胞内胰蛋白酶、淀粉酶活性.结果:模型组较健康组大鼠血清IgA、IgG、IgM降低,胰腺p38MAPK、ATF2及腺泡细胞中胰蛋白酶、淀粉酶升高;与模型组相比,Octreotide组血清IgA、IgG、IgM升高,胰腺p38MAPK、ATF2及腺泡细胞中胰蛋白酶、淀粉酶降低;与Octreotide组相比,HFE+Octreotide组血清IgA、IgG、IgM升高,胰腺p38MAPK、ATF2及腺泡细胞中胰蛋白酶、淀粉酶降低.模型组胰腺组织及腺泡细胞损伤较严重;Octreotide组病变、细胞数量及形态均有所改善,但仍有结构损伤;HFE+Octreotide组细胞变性肿胀减少,胰腺组织逐渐恢复.结论:HFE联合Octreotide可治疗胰腺炎大鼠,修复腺泡细胞损伤,改善免疫指标,作用机制与调节p38MAPK、ATF2水平相关.
Objective:To investigate the effects of Honeysuckle extract(HFE)combined with Octreotide on acinar cell injury repair,immune indices and p38MAPK/ATF2 levels in rats with severe pancreatitis.Methods:Forty rats were divided into healthy group,model group,HFE+Octreotide group,and Octreotide group.Severe pancreatitis models were established in all groups except the healthy group.The healthy group and model group were intraperitoneally injected with 1 mL/kg saline;the HFE+Octreotide group was injected with 120 mg/kg HFE and 10 μg/kg Octreotide via the tail vein;and the Octreotide group was injected with 10 μg/kg Octreotide via the tail vein.All injections were administered once daily for a total of four weeks.Immunoglobulins(IgA,IgG,IgM)were detected by enzyme-linked immunosorbent assay(ELISA).Immunohistochemistry was used to detect the p38 mitogen-activated protein kinase(p38MAPK)and activating transcription factor 2(ATF2)in rat pancreatic tissue.H-E staining was used to observe the pathological changes of pancreatic tissue.Protease and amylase activities were measured within acinar cells by using colorimetric method.Results:Compared with healthy group,the model group showed a decrease in serum levels of IgA,IgG and IgM,an increase in p38MAPK,ATF2,protease and amylase activities within acinar cells.Compared with the model group,the Octreotide group exhibited an increase in serum levels of IgA,IgG,IgM,a decrease in p38MAPK,ATF2,protease and amylase activities within acinar cells.Compared with the Octreotide group,the HFE+Octreotide group showed an increase in serum levels of IgA,IgG,IgM,and a decrease in p38MAPK,ATF2,protease and amylase activities within acinar cells.The model group exhibited more severe tissue damage and acinar cell injury.The Octreotide group showed improvements in lesion severity,cell quantity,and morphology,but structural damage was still present.In HFE+Octreotide group,cell degeneration and swelling were decreased,with gradual restoration of pancreatic tissue.Conclusion:Honeysuckle extract combined with Octreotide can treat severe pancreatitis in rats by repairing acinar cell damage and improving immune indices,the mechanism of which is related to the regulation of p38MAPK and ATF2 levels.
刘斐;姚杰;张丽骞
河北北方学院附属第一医院,重症医学科,张家口 075000河北北方学院附属第一医院,麻醉科,张家口 075000
胰腺炎;金银花提取物;奥曲肽;腺泡细胞;免疫;p38丝裂原活化蛋白激酶
severe pancreatitis;Honeysuckle extract;Octreotide;acinar cells;immune;p38 mitogen-activated protein kinase
《解剖学杂志》 2023 (006)
497-501,524 / 6
河北省医学科学研究基金(20200524)
10.3969/j.issn.1001-1633.2023.06.006
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