目的 探究微小RNA-133a-3p(miR-133a-3p)通过靶向调控CAND蛋白1(CAND1)对乳腺癌细胞侵袭及凋亡的影响及机制.方法 MCF-7细胞分为过表达组(转染mimics-miR-133a-3p)、NC组(转染mimics对照)、共转染组(共同转染mimics-miR-133a-3p和pcDNA-CAND1)、对照组(仅加入等量转染试剂).以流式细胞仪检测细胞凋亡情况,以Transwell实验检测细胞侵袭能力,以实时荧光定量聚合酶链反应检测miR-133a-3p和CAND1的表达.结果 转染后,对照组、NC组、过表达组及共转染组miR-133a-3p表达水平分别为 0.50±0.08、0.51±0.09、1.06±0.10 和 1.05±0.15,CAND1 mRNA的表达分别为 0.91±0.09、0.91±0.07、0.80±0.10 和 1.21±0.10.上述指标,共转染组与对照组、NC组比较,在统计学上差异均有统计学意义(均P＜0.05),过表达组与对照组、NC组比较,在统计学上差异均有统计学意义(均P＜0.05).对照组、NC组、过表达组及共转染组中细胞凋亡率分别为(7.88±1.62)％、(8.87±2.01)％、(53.41±5.46)％和(29.54±3.78)％;对照组、NC 组、过表达组及共转染组中细胞侵袭数分别为(161.02±10.31)、(155.87±12.30)、(85.21±9.11)和(118.37±10.84)个.上述指标,转染组与过表达组、对照组、NC组比较,在统计学上差异均有统计学意义(均P＜0.05),过表达组与对照组、NC组比较,在统计学上差异均有统计学意义(均P＜0.05).结论 在人乳腺癌细胞MCF-7中过表达miR-133a-3p可抑制CAND1,促进MCF-7细胞凋亡和侵袭.
Objective To explore the effect and mechanism of microRNA-133a-3p(miR-133a-3p)on invasion and apoptosis of breast cancer cells through targeted regulation of cullin-associated NEDD8-dissociated 1(CAND1).Methods MCF-7 cells were divided into overexpression group(mimics miR-133 a-3p transfection),NC group(mimics control transfection),co-transfection group(mimics miR-133a-3p transfection with pcDNA-CAND1 co-transfection)and control group(only adding the same amount of transfection reagents).Flow cytometry was used to detect cell apoptosis,Transwell assay was used to detect cell invasion,and real-time fluorescence quantitative polymerase chain reaction was used to detect miR-133a-3p and CAND1 expression.Results After transfection,the expression levels of miR-133a-3p in control group,NC group,overexpression group and co-transfection group were 0.50±0.08,0.51±0.09,1.06±0.10 and 1.05±0.15,respectively;the expression of CAND1 mRNA were 0.91±0.09,0.91±0.07,0.80±0.10 and 1.21±0.10,respectively.There were statistically significant differences in the above indexes between the co-transfection group and the control group,the NC group(all P＜0.05),and there were statistically significant differences between the overexpression group and the control group,the NC group(all P＜0.05).The apoptosis rates in control group,NC group,overexpression group and co-transfection group were(7.88±1.62)％,(8.87±2.01)％,(53.41±5.46)％,(29.54±3.78)％,respectively.The number of invasive cells in control group,NC group,overexpression group and co-transfection group were 161.02±10.31,155.87±12.30,85.21±9.11 and 118.37±10.84,respectively.There were statistically significant differences in the above indexes between transfection group and overexpression group,control group and NC group(all P＜0.05),and there were statistically significant differences between overexpression group and control group and NC group(all P＜0.05).Conclusion Overexpression of miR-133a-3p in human breast cancer cells MCF-7 can inhibit CAND1 and promote apoptosis and invasion of MCF-7 cells.
田彩平;闵建平;荆晓;杨碎胜
甘肃省医学科学研究院医学分子生物学研究中心,甘肃兰州 730050甘肃省医学科学研究院转化医学研究中心,甘肃兰州 730050甘肃省肿瘤医院核医学科,甘肃兰州 730050甘肃省肿瘤医院乳腺一科,甘肃兰州 730050
药学
微小RNA-133a-3p;乳腺癌;侵袭;凋亡
microRNA-133 a-3p;breast cancer;invade;apoptosis
《中国临床药理学杂志》 2024 (001)
37-41 / 5
甘肃省卫生行业科研计划基金资助项目(GSWSKY-2019-35);甘肃省科技计划基金资助项目(22JR5RA643);甘肃省卫生行业科研管理基金资助项目(GSWSKY-2019-96);兰州市科技发展指导性计划基金资助项目(2020-ZD-52)
10.13699/j.cnki.1001-6821.2024.01.008
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