野西瓜多糖上调LINC00476对胰腺癌细胞增殖、迁移、侵袭的影响OACSTPCD

Objective To investigate the effect of Capparis spionosa polysaccharides on the proliferation,migration and invasion of pancreatic cancer cells by regulating LINC00476.Methods Pancreatic cancer cells SUIT-2 were divided into blank group and experimental-L,-M,-H groups,which were treated with 0,200,400,600 μg·mL-1 Capparis spionosa polysaccharides,respectively.Clone formation experiments were used to detect cell proliferation ability,Transwell experiments were used to detect cell migration and invasion ability,real-time fluorescent quantitative polymerase chain reaction was used to detect the expression of LINC00476 and human antigen R(HuR)mRNA,and Western blot was used to detect the expression levels of HuR proteins.Results The colony formation number in blank group and experimental-L,-M,-H groups were 137.31±9.43,109.57±4.72,77.28±5.76 and 50.74±6.76;the number of migrating cells were 229.21±27.67,188.69±9.29,150.98±20.25 and 109.37±8.04;the number of invasive cells were 125.96±9.20,100.52±7.60,81.65±8.62 and 66.01±5.79;the expression levels of LINC00476 mRNA were 0.96±0.07,1.38±0.16,1.68±0.18 and 2.18±0.30;the expression levels of HuR mRNA were 1.05±0.07,0.81±0.06,0.58±0.10 and 0.32±0.04;the relative expression levels of HuR protein were 1.00±0.09,0.74±0.05,0.55±0.03 and 0.32±0.02.Compared with blank group,the proliferation,migration and invasion abilities of experimental-L,-M,-H groups were decreased,the expression levels of HuR were decreased,and the expression levels of LINC00476 mRNA were increased,the differences were statistically significant(all P＜0.05).Conclusion Capparis spionosa polysaccharides may inhibit the proliferation,migration and invasion of pancreatic cancer cells by up-regulating LINC00476.