UPLC-MS/MS法同时测定蛋白饮料和液体调味品中36种防腐剂OACSTPCD

An ultra-performance liquid chromatography-electrospray ionization tandem mass spectrometry(UPLC-MS/MS)method was developed and optimized for the simultaneous determination of 36 preservatives in protein drinks and liquid condiments by the pretreat-ment method of combining extraction and purification.The samples were purified with saturated sodium chloride solution(adjusting pH to 3 with phosphoric acid)and extracted with acetonitrile-methanol(9∶1,volume ratio)(containing 0.2％formic acid).The analytes were separated on a C18 chromatographic column using gradient elution with methanol-5 mmol/L ammonium acetate as the mobile phase,detec-ted by MS/MS in both positive ion and negative ion mode using dynamic multiple reaction monitoring,and finally quantified by the matrix-matched external standard method.Results showed that good linearity was observed for the 36 compounds with correlation coefficients≥0.999 in the concentration range of 1-250 ng/mL,and the limits of quantitation were between 0.04 mg/kg and 0.2 mg/kg.The average recoveries of blank samples were in the range of 75％-119％,with relative standard deviations of 0.90％-9.8％.The established high-throughput detection method is sensitive,rapid,accurate,and simple,can effectively reduce matrix interference,reduce detection cost,shorten the detection cycle,and greatly improve the qualitative and quantitative detection efficiency of various preservatives.