为了比较牡蛎酶解提取物(enzymolysis extract of oyster,EPO)、牡蛎肽(oyster peptide,OP)和牡蛎水提取物(oyster water extract,WPO)的抗衰老作用,首先进行了基本成分的测定,继而以秀丽隐杆线虫(Caenorhabdi-tis elegans)为动物模型,研究3种牡蛎提取物对线虫的寿命、生殖、脂褐素积累、运动能力、急性氧化应激损伤及体内氧化还原水平等指标的影响.结果表明,EPO、OP和WPO的基本成分有一定的差异,但主要成分均为蛋白质,其中OP的总蛋白质含量最高,为(66.59±0.57)g/100 g干基;与空白组相比,低中高浓度(50、200、800 μg/mL)的EPO、OP和WPO均能延长线虫寿命,但低浓度作用不显著,仅OP能呈浓度依赖性地显著增加线虫的产卵量(P＜0.05),最高可增加80.2％;3种提取物均能显著降低线虫体内脂褐素的积累(P＜0.05),并呈剂量依赖性,最高可达98.15％;高浓度的3种提取物均能显著改善衰老线虫的运动能力(P＜0.05),还能显著提高百草枯损伤线虫的存活率及线虫体内谷胱甘肽过氧化物酶和超氧化物歧化酶活力,降低丙二醛含量(89.2％-98.2％)(P＜0.05),其作用大小顺序为OP＞EPO＞WP O.因此,3种不同牡蛎提取物均表现出抗衰老作用,但作用强度有差异,OP的表现最好,研究结果可为进一步开发牡蛎抗衰老食品提供参考.
To compare the anti-aging effects of two oyster enzymatic extracts,including enzymolysis extract of oyster(EPO),oyster peptide(OP),and oyster water extract(WPO),the basic components of the three oyster extracts were determined,and Caenorhabditis elegans was used as an animal model to study the effects of oyster extracts on lifespan,reproduction,lipofuscin accumulation,athletic ability,acute oxidative stress injury,and redox level in the body.Results showed that the basic components of EPO,OP,and WPO were different,but the main components were all proteins.Among them,OP had the highest total protein content with(66.59±0.57)g/100 g dry basis.Compared with control,the low,medium,and high concentrations(50,200,800 μg/mL)of EPO,OP,and WPO prolonged C.elegans lifespan,but the effect was not significant at low concentrations.Between three oyster extracts,only OP significantly increased the egg production of C.elegans in a concentration-dependent manner(P＜0.05),with a maximum increase of 80.2％.All three ex-tracts significantly reduced the lipofuscin accumulation in C.elegans(P＜0.05)in a dose-dependent manner with a maximum of 98.15％.High concentrations of three oyster extracts(800 μg/mL)significantly improved the movement ability of aging C.elegans,in-creased the survival rate of C.elegans damaged by paraquat(P＜0.05)as well as the activity of GSH-Ps and SOD enzymes,and reduced MDA contents in vivo by 89.2％to 98.2％(P＜0.05).The effects of three oyster extracts had the order of OP＞EPO＞WPO.In con-clusion,EPO,OP,and WPO showed different anti-aging effects.OP showed the best effect,which could provide a reference for further development of oyster foods for anti-aging.
魏润霞;卢虹玉;秦小明;王丽瑶
广东海洋大学食品科技学院,广东省水产品加工与安全重点实验室,广东省海洋生物制品工程实验室,广东省海洋食品工程技术研究中心,水产品深加工广东普通高等学校重点实验室,广东湛江,524008广东海洋大学食品科技学院,广东省水产品加工与安全重点实验室,广东省海洋生物制品工程实验室,广东省海洋食品工程技术研究中心,水产品深加工广东普通高等学校重点实验室,广东湛江,524008||大连工业大学,海洋食品精深加工关键技术省部共建协同创新中心,辽宁大连,116034
牡蛎提取物;秀丽隐杆线虫;脂褐素;百草枯;抗衰老
oyster extract;Caenorhabditis elegans;lipofuscin;paraquat;anti-aging
《食品与发酵工业》 2024 (001)
52-58 / 7
财政部和农业农村部国家现代农业产业技术体系资助项目(CARS-49)
10.13995/j.cnki.11-1802/ts.037222
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