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刺五加黑斑病生防细菌分离、筛选及室内防控效果OACSTPCD

Isolation,screening and indoor control effect of biocontrol bacteria against Acanthopanax senticosus black spot

中文摘要英文摘要

[目的]筛选对刺五加黑斑病具有生防潜力的细菌菌株.[方法]采用平板对峙法从健康刺五加根际土壤中分离筛选对刺五加黑斑病菌具有拮抗作用的菌株,基于形态学、生理生化鉴定及 16S rDNA结合gyrB测序分析进行菌种分类鉴定,测定其抗菌谱;并通过叶面定殖及盆栽防效试验进一步评价该菌的生防潜力.[结果]分离得到 419 株细菌,其中,菌株YZ-375 鉴定为贝莱斯芽孢杆菌Bacillus velezensis.菌株YZ-375 对刺五加黑斑病菌的拮抗效果较好,无菌滤液的抑制率为 65.85%,且对人参立枯丝核菌Rhizoctonia solani、人参链格孢菌Alternaria panax、人参茄腐皮镰孢菌Fusarium solani、人参核盘菌Sclerotinia ginseng、人参毁灭柱孢菌Cylindrocarpon destructans、五味子尖镰孢菌Fusarium oxysporum均具有拮抗效果,抑制率为 69.21%~77.44%.在鲜叶定殖 35d后,菌株YZ-375 菌量仍能保持在每克鲜叶 103 CFU.菌株YZ-375 对刺五加黑斑病防效达72.31%,与枯草芽孢杆菌、苯醚甲环唑防效无显著差异.[结论]贝莱斯芽孢杆菌YZ-375 具有良好的生防潜力,具有一定的开发应用前景.

[Objective]In order to screen the bacteria with biological control potential against Acanthopanax senticosus black spot.[Method]The bacteria with antagonistic effect against A.senticosus black spot were isolated and screened from the rhizosphere soil of healthy A.senticosus by plate confrontation method.The screened strains was classified and identified based on morphological,physiological,biochemical identification and 16S rDNA combined with gyrB sequencing analysis,and the antibacterial spectrum was determined.Its biocontrol potential was further evaluated through leaf colonization and pot control effect test.[Result]A total of 419 strains of rhizosphere bacteria were isolated from A.senticosus.Among them,the strain YZ-375 was identified as Bacillus velezensis.Strain YZ-375 had good antagonistic effect against the black spot pathogen of A.senticosus,the inhibition rate of sterile filtrate was 65.85%.The strain YZ-375 had antagonistic effects against Rhizoctonia solani,Alternaria panax,Fusarium solani,Sclerotinia gingseng,Cylindrocarpon destructans and Fusarium oxysporum,with the inhibition rates from 69.21%to 77.44%.After 35 d of colonization in fresh leaves,the bacterial count of strain YZ-375 remained at 103 CFU per gram of fresh leaves.The control effect of strain YZ-375 on black spot of A.senticosus reached 72.31%,which was not significantly different from that of Bacillus subtilis and difenoconazole.[Conclusion]B.velezensis YZ-375 has good biocontrol potential and has certain development and application prospects.

丁艳哲;杜立财;孙卓;杨利民;韩忠明;王云贺;刘子君;张浩

吉林农业大学中药材学院/省部共建生态恢复和生态系统管理国家重点实验室培育基地,吉林长春 130118吉林参王植保科技有限公司,吉林白山 134504吉林吉隆东北沙棘产业有限责任公司 吉林大安 131300辽宁盘锦检验检测中心,辽宁盘锦 124010

植物保护学

刺五加;黑斑病;贝莱斯芽孢杆菌;分类鉴定;叶面定殖;生物防治

Acanthopanax senticosus;Black spot;Bacillus velezensis;Classification and identification;Leaf colonization;Biological control

《华南农业大学学报》 2024 (002)

266-272 / 7

吉林省科技发展计划项目(20200708033YY);中央财政林业科技推广示范项目(JLT2021-34);现代农业产业技术体系建设项目(CARS-21)

10.7671/j.issn.1001-411X.202302013

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