阴道加德纳菌与阴道阿托波菌共培养生长状态及粪肠球菌上清液对两者共培养抑制作用的体外研究OACSTPCD

Objective:To study the growth characteristics of Gardnerella vaginalis and Atopobium vaginalis co-cultured,and to explore the effect of Enterococcus faecalis supernatant on biofilm formation in mixed culture of Gardnerella vaginalis and Atopobium vaginalis.Meth-ods:Gardnerella vaginalis and Atopobium vaginalis isolated and identified from vaginal secre-tion samples of BV patients,and the growth and film formation of Gardnerella vaginalis and Atopobium vaginalis co-cultured for 24h,48h and 72h were observed.The growth and acid-pro-ducing ability of Enterococcus faecalis standard strain within 24 hours were observed.The bio-film formation of Gardnerella vaginalis after single culture,mixed culture of Gardnerella vagina-lis and Atopobium vaginalis and co-culture with supernatant of Enterococcus faecalis for 24 hours,48 hours and 72 hours were observed.Results:The film-forming ability of Gardnerella sp.and Atobo sp.co-cultured in the ratio of 2:1 was stronger than that of Gardnerella sp.with the same amount of bacteria,and the difference was statistically significant(P<0.05).Atopobi-um bacteria hardly produce biofilm.The supernatant of Enterococcus faecalis significantly inhib-ited the biofilm formation of Gardnerella simplex and mixed culture of Gardnerella and Atopobi-um(2:1),and the difference was statistically significant(P<0.05).Conclusions:The co-culture of Gardnerella vaginalis and Atopobium vaginalis can promote the formation of biofilm.The supernatant of Enterococcus faecalis can inhibit the biofilm formation of Gardnerella vagina-lis and Gardnerella vaginalis and Atopobium vaginalis.