杉木不同世代育种群体遗传多样性评价及核心种质构建
The evaluation of the genetic diversity of different generations of breeding populations and establishing core collections of Chinese fir
[目的]评价湖南省杉木不同世代育种群体传多样性及其变化动态,构建核心种质,为湖南省杉木高世代遗传改良以及种质资源科学利用提供理论依据。[方法]采用SSR标记技术评价杉木3 个不同世代育种群体遗传多样性,Gene Marke r 软件进行基因分型,GenAlex 估算群体遗传参数、分子方差分析和主成分分析,FSTAT 评估成对育种群体间的遗传分化程度,STRUCTURE分析群体遗传结构,Core-hunter构建核心种质集,t检验和主坐标分析法验证核心种质的有效性和代表性。[结果]湖南省杉木育种群体遗传多样性丰富(HO=0.541,HE=0.630),3个不同世代育种群体均呈现出极度不平衡状态,表现为杂合体缺失;不同世代育种群体间遗传分化小(FST在0.01～0.014之间),种质材料的遗传背景较为复杂;筛选出55 份核心种质集的覆盖度与代表性良好。[结论]湖南省杉木育种群体遗传多样性较高,高世代育种群体遗传多样性没有明显下降,表明湖南省杉木多世代遗传改良策略与方法科学有效。筛选的核心种质具有较好的代表性,可为湖南杉木种质资源高效利用及高世代种子园建设提供优异的种质材料。
[Objective]In order to evaluate the diversity and genetic structure of the three generations of Chinese fir(Cunninghamia lanceolata(Lamb.)Hook)breeding groups in Hunan,build a cedar core germplasm,and provide scientific basis for the breeding and utilization of the cedar species and use,21 pairs of SSR markings of fir clone parent from three generations were used to evaluate genetic diversity and genetic structural evaluation.Use the A-NE and CV methods to build core germplasm.[Result]The overall genetic diversity of the three-generation breeding groups of fir is high(observation hybridness(HO)is 0.541,and the expected hybridness(HE)is 0.630).The second-generation breeding group has the highest genetic parameters,and the observation hybridness(HO)and the expectation hybridness(HE)are 0.575 and 0.639;the Fst of three fir breeding group is between 0.01～0.014,and genetic diversity mainly exists in the group,only 2%between groups,and the degree of genetic differentiation between groups is low;55 core germplasms were selected.[Conclusion]The overall genetic diversity of the three generations of Chinese fir breeding groups in Hunan is high.The genetic parameters of the three groups have changed less,and the degree of genetic differentiation between groups is low.The resources of Hunan Chinese fir Breeding Group are the most suitable to divided into two groups.The screened core breeding group provided a scientific basis for reasonable preservation and efficient utilization of Hunan Chinese fir resources and seed orchard quality transformation
张勰;伍汉斌;黄帆;程勇;蒋宏春;张森强;王欣;段爱国;
湖南省林业科学院,长沙 410004 中国##中南林业科技大学林学院,长沙 410004 中国;中国林业科学研究院林业研究所,北京 100091 中国;湖南省林业科学院,长沙 410004 中国;湖南省林业科学院,长沙 410004 中国;会同县广坪国有林场,湖南 会同 418307 中国;会同县广坪国有林场,湖南 会同 418307 中国;湖南省林业科学院,长沙 410004 中国##中南林业科技大学林学院,长沙 410004 中国;中国林业科学研究院林业研究所,北京 100091 中国;
Chinese fir breeding group genetic diversity core germplasm
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