香榧花粉萌发油体相关蛋白鉴定及功能初探

Torreya grandis is one of China's unique and precious economic tree species,belonging to the cultivated type of the gymnosperm family Taxaceae.The kernels of Torreya grandis are considered as rare nuts due to their high nutritional value,distinctive flavor,and various biological functions,including antioxidant,cough-suppressant,anti-inflammatory,antimicrobial,and anti-tumor properties.However,Torreya grandis trees are typically dioecious,with male and female trees maturing at different times,necessitating artificial pollination.Consequently,the pollen activity significantly affects the yield and quality of Torreya grandis kernels.Currently,pollen activity detection relies mainly on staining methods,which may not accurately reflect pollen activity.Furthermore,the growth and development of the pollen tube require the coordinated action of vesicles and numerous organelles,which demands a continuous energy supply.Lipid bodies are commonly present in pollen,participating in pollen germination and providing energy.This study obtained pollen with high germination rates through in vitro cultivation and explored the mechanism of lipid bodies'involvement in pollen germination by studying their dynamics and proteomics identification.Results:(1)This study initially screened the in vitro pollen germination medium for Torreya grandis,and through orthogonal experiments,identified the optimal culture medium and conditions.Additionally,it was found that plant growth regulators NAA,GA3,and 2,4-D could promote Torreya grandis pollen germination at low concentrations while inhibiting germination at high concentrations.(2)Morphological observations of Torreya grandis pollen revealed distinct lateral germination features,further confirmed by in vivo paraffin sections.(3)Tracking the dynamics of vesicles and lipid bodies during pollen germination revealed that their movement rates were considerably slower than those in other gymnosperms and closely related to the pollen tube growth rate.(4)Stable extraction of pollen germination lipid bodies,after purity identification,allowed for an initial exploration of the mechanism of lipid bodies'involvement in pollen germination from a proteomics perspective.