核桃原生质体瞬时转化及油体蛋白功能初探

Walnut(Juglans regia L.),as a vital economic tree species,has gained significant attention,primarily due to its high fat content.The high nutritional value,mild taste,and easy digestibility of walnut oil make it an ideal choice for premium health-promoting edible oil,especially suitable for children during growth,women during pregnancy,and postpartum recovery.However,as a perennial woody plant,its long fruiting cycle,nutrient depletion during male flower development,and other physiological characteristics significantly hinder the development of the walnut industry.Fast breeding,improvement,and breeding are research hotspots in this context.Currently,the research system for transient genetic transformation and protein subcellular localization in walnuts is not well-established.Transient transformation and stable expression techniques can be used to achieve the rapid expression of target genes in recipient cells,providing a rapid and efficient approach for subsequent studies of walnut gene-protein relationships.Oil bodies are critical organelles in plant cells responsible for storing fats and oils.This function is crucial for supporting seed energy and nutrient reserves,promoting seedling growth and development.Investigating oil bodies can optimize the production pathway of walnut oil,enhancing both yield and quality.In this context,we conducted research on the transient transformation system of walnuts and the subcellular localization of oil body proteins.The results showed that:(1)This study established a transient transformation system,providing a new tool for walnut research.(2)We screened a set of walnut organelle marker proteins,validating the feasibility and accuracy of the transient transformation system.(3)Through bioinformatics analysis,we identified the Oleosins gene family members,exploring their physical properties and evolutionary relationships,and confirmed their subcellular localization using the transient transformation system.