桉树枝瘿姬小蜂体内细菌对其寿命及酶活性影响
Effects of Bacterial Endosymbionts on the Lifespan and Enzyme Activities of Leptocybe invasa
[目的]探索可培养内生细菌对宿主桉树枝瘿姬小蜂(Leptocybe invasa)的寿命和酶活性的影响，为研究相关细菌的基因功能和研制桉树枝瘿姬小蜂生物抑制剂防打下基础。[方法]以新羽化的桉树枝瘿姬小蜂雌虫以及前期从其体内分离出的10种优势内生菌(LI01、LI03、LI05、LI07、LI09、LI10、LI12、LI13、LI14和LI16)为材料，利用纯化后培养的细菌以配制不同含菌液，以饲喂10％的蜂蜜水为对照(CK)，观察各组小蜂的寿命情况；并挑选出对桉树枝瘿姬小蜂雌虫寿命具有延长、缩短和无影响的菌株各两株，分别收集处理1d、3d、6d和9d后的雌虫用于保护酶、解毒酶和转氨酶活性的测定。[结果]CK组桉树枝瘿姬小蜂寿命为11.60±1.52d，饲喂LI01、LI14和LI07菌株的桉树枝瘿姬小蜂寿命延长，分别为14.27±0.85d、14.17±0.68d和13.50±0.68d，分别延长了约2.67d、2.57d、1.9d；饲喂LI10、LI16和LI09菌株的桉树枝瘿姬小蜂寿命缩短，分别为3.83±0.46d、3.50±0.18d和3.5±0.31d，分别缩短了约7.77d、8.1d和8.1d；其余菌株对桉树枝瘿姬小蜂寿命无显著影响。饲喂对桉树枝瘿姬小蜂寿命有缩短作用的LI09和LI10含菌液后，桉树枝瘿姬小蜂体内的保护酶(SOD、CAT、POD)、解毒酶(AchE、GST)和转氨酶(AST、ALT)活性，在第一天显著高于CK组的酶活性，而第三天时低于CK组，总体上都呈现出先升高后降低的趋势；而饲喂对桉树枝瘿姬小蜂寿命有延长作用的LI01、LI14含菌液和缩短作用的LI03和LI05含菌液后，其保护酶和解毒酶活性在第1d、3d和6d时都与与CK相比无显著区别，只有在饲喂含菌液的第9d时，其转氨酶活性显著高于CK。[结论]饲喂泛菌(Pantoea anthophila)LI01、单胞菌(Stenotrophomonas sp.)LI07和葡萄球菌(Staphylococcus sp.)LI14对宿主寿命有延长的效果，饲喂沙雷氏菌(Serratia marcescens)LI09、克雷伯氏菌(Klebsiella pneumoniae)LI10和肠杆菌(Enterobacter cloacae)LI16会降低宿主寿命。饲喂LI09和LI10菌株后，桉树枝瘿姬小蜂体内的保护酶、解毒酶和转氨酶发生显著改变，说明此类沙雷氏菌和克雷伯氏菌能够影响宿主酶活性从而影响宿主的代谢与寿命。
[Objective]:To explore the effects of culturable endosymbiotic bacteria on the lifespan and enzyme activities of the host Leptocybe invasa(L.invasa),providing a foundation for the study of related bacterial gene functions and the development of biological inhibitors for L.invasa control.[Methods]:Using newly emerged female L.invasa and ten dominant endosymbiotic bacteria(strains LI01,LI03,LI05,LI07,LI09,LI10,LI12,LI13,LI14,and LI16)previously isolated from their bodies,purified and cultured bacteria were used to prepare different bacterial suspensions.A 10%honey water solution served as the control(CK).The lifespan of the wasps was observed after feeding them with different bacterial suspensions.Two strains that extended,shortened,and had no effect on the lifespan of female L.invasa were selected.Female wasps collected at 1 d,3 d,6 d,and 9 d after treatment were used to determine the activities of protective enzymes(SOD,CAT,POD),detoxifying enzymes(AchE,GST),and transaminases(AST,ALT).[Results]The lifespan of L.invasa in the CK group was 11.60±1.52 d.Feeding strains LI01,LI14,and LI07 increased the lifespan to 14.27±0.85 d,14.17±0.68 d,and 13.50±0.68 d,respectively,an increase of approximately 2.67 d,2.57 d,and 1.9 d.Feeding strains LI10,LI16,and LI09 decreased the lifespan to 3.83±0.46 d,3.50±0.18 d,and 3.5±0.31 d,respectively,a decrease of approximately 7.77 d,8.1 d,and 8.1 d.Other strains had no significant effect on the lifespan of L.invasa.After feeding L.invasa with bacterial suspensions containing strains LI09 and LI10,which shorten the wasp's lifespan,the activities of protective enzymes(SOD,CAT,POD),detoxifying enzymes(AchE,GST),and transaminases(AST,ALT)were significantly higher than those in the CK group on day 1,but lower on day 3,showing an overall trend of first increasing then decreasing.In contrast,when feeding bacterial suspensions containing strains LI01 and LI14,which extend the wasp's lifespan,and strains LI03 and LI05,which also shorten the lifespan,the activities of protective and detoxifying enzymes at days 1,3,and 6 showed no significant difference compared to the CK group.Only on day 9 after feeding with the bacterial suspensions did the transaminase activity show a significant increase over the CK group.[Conclusion]Feeding Pantoea anthophila strain LI01,Stenotrophomonas sp.strain LI07,and Staphylococcus sp.strain LI14 extends the host's lifespan,while feeding Serratia marcescens strain LI09,Klebsiella pneumoniae strain LI10,and Enterobacter cloacae strain LI16 reduces the host's lifespan.After feeding strains LI09 and LI10,there are significant changes in the activities of protective enzymes,detoxifying enzymes,and transaminases in L.invasa,indicating that these Serratia and Klebsiella strains can affect host enzyme activity,thereby influencing the host's metabolism and lifespan.
周乐明;
广西大学 南宁 530004;
Leptocybe invasa endosymbionts longevity Protective enzymes Detoxifying enzymes Transaminases
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