阿拉善平颜蚜蝇对肉苁蓉不同器官挥发物的选择行为

[Objective]Eumerus alxaensis Huo&Liu is a borescent pest that mainly harms Cistanche deserticoia Y.C.Ma.Considering that its host is a pharmacological plant,it is important to explore appropriate biological control measures.In this study,volatile substances in inflorescences and fleshy stems that have attractants to E.alxaensis in different physiological states were screened and the optimal concentrations were selected,aiming to provide theoretical basis for the research and development of attractants of E.alxaensis.[Methods]The relatively high content of 8 volatile compounds in C.deserticoia Y.C.Ma inflorescence and fleshy stem were measured by Y-shaped olfactometry before mating of male and female adults and in early oviposition stage of female.On the basis of the selective behavior reaction under three concentrations(stock solution,10％solution and 1％solution),field lure tests of the monomer with optimum volatile concentration were conducted in Haloxylon ammodendron(C.A.Mey.)Bunge combined with yellow plate.[Results]The results of laboratory experiments showed that the adults of E.alxaensis had strong positive taxis to the volatiles of 8 inflorescence and fleshy stems.The reaction rate of female adults to 1％benzaldehyde and caryophyllene was 50.00％and 48.30％,respectively,and the reaction rate to cetane in inflorescence volatiles was 38.30％,and the interaction between different volatiles and concentrations was significant.The reaction rate of male adult worms to caryophyllene and eugenol was 60.00％and 51.7％respectively,and the reaction rate to 1-chlorododecyl in inflorescence volatiles was 43.30％,and the interaction between different volatiles and concentrations was significant.Before laying eggs,the reaction rates of female to caryophyllene and benzaldehyde were 48.40％and 46.70％,respectively,and the reaction rates of limonene and cetane in inflorescence volatiles were 38.30％and 36.70％,respectively,and there was no significant interaction difference among different volatiles and concentrations.Field experiments showed that caryophyllene and eugenol in the succulent stem volatiles had better trapping effect on E.alxaensis.The average trapping quantity of caryophyllene and eugenol in the succulent stem volatiles was 13.50/piece and 13.60/piece,respectively,respectively.The trapping effect of limonene in the inflorescence volatiles was better.The average trapping amount of 3 days was 9.30/piece.The trapping amount of the above three volatile substances was significantly higher than that of CK.Among them,caryophyllene had a stronger trapping effect on female than male(P=0.03),and the average trapping number of male was 7.3/piece.Eugenol and limonene had a stronger trapping effect on male,and the average trapping number of male was 9.12/piece and 6.08/piece,respectively.[Conclusion]The results show that eugenol and caryophyllene can be used as the first choice compounds for the attractant of E.alxaensis.