闽楠DOF基因家族的鉴定和表征及其在非生物胁迫——干旱、高温和光胁迫中的作用
Identification and characterization of the DOF gene family in Phoebe bournei and its role in abiotic stress—Drought,Heat and Light stress
[目的]明确闽楠中Dof转录因子基因家族成员，并且分析得出闽楠Dof基因家族在响应非生物胁迫中起的作用。[方法]本研究在全基因组尺度上对闽楠Dof基因家族进行了综合测定和分析，包括其系统发育关系、基因结构、蛋白质的理化性质、顺式作用元件、共线性和共线分析、Ka(非同义替换率)、Ks(同义替换率)和Ka/Ks(omega(ω))值、组织表达谱、SSR位点预测和非生物下实时定量PCR测定基因相对表达水平。[结果]在这项研究中，我们在全基因组水平上鉴定并分析了34个PbDof基因成员。结果表明，34个PbDof基因在12条染色体上分布不均匀。我们利用来自闽楠和拟南芥的Dof基因构建了系统发育树，并将这些基因分为8个亚组。在种间共线性分析中，AtDof和Pb Dof之间存在16个同源基因对，ZmDof和PbDof之间存在9个同源基因对。我们还进行了顺式作用元件分析，发现参与光响应的顺式作用元件在PbDof基因中最丰富。通过SSR位点预测，我们根据结果得出闽楠Dof基因的进化水平较低。此外，我们进行qRT-PCR实验评估了8个PbDof基因在高温、干旱和光照胁迫下的表达谱。特别是，PbDof08和PbDof16在3种胁迫下显著上调。[结论]闽楠是中国特有的二级濒危保护物种，具有重要的生态和经济价值。DNA结合单锌指(Dof)转录因子是植物特异性调节因子。大量研究表明，Dof基因参与植物的生长、发育和对非生物胁迫的反应。我们通过系统发育分析，发现PbDof11与AT5G60850.1表现出高度同源性，而PbDof07与AT3G50410.1表现出高度同源性。基因共线性分析结果表明，34个PbDof基因形成了24对同源基因对。在干旱、高温和光照处理下，分析7个PbDof基因的表达模式。推测PbDof08和PbDof16是参与光响应的主要基因。此外，还讨论了植物处于非生物胁迫下Dof基因的作用机制。本研究为PbDof基因研究提供了基础信息，并为进一步研究Dof转录因子响应逆境的机制以及闽楠Dof基因家族对环境变化的适应提供了新的见解。
[Objective]The members of the Dof transcription factor gene family in Phoebe bournei were clarified,and the role of Dof gene family in response to abiotic stress was analyzed.[Method]In this study,the Dof gene family was comprehensively determined and analyzed at the genome-wide scale,including its phylogenetic relationship,gene structure,physicochemical properties of proteins,cis-acting elements,collinearity and collinear analysis,Ka(non-synonymous replacement rate),Ks(synonymous replacement rate)and Ka/Ks(omega(ω)＞values,tissue expression profile,SSR locus prediction,and real-time quantitative PCR to determine the relative expression levels of genes.In this study,we identified and analyzed 34 PbDof gene members at the whole-genome level.[Result]The results indicated that the 34 PbDof genes were unevenly distributed across 12 chromosomes.We utilized the Dof genes from Arabidopsis thaliana and P.bournei to construct a phylogenetic tree and categorized these genes into eight subgroups.In the collinearity analysis,there were 16 homologous gene pairs between AtDof and PbDof and nine homologous gene pairs between ZmDof and PbDof.We conducted a cis-acting element analysis and found that cis-acting elements involved in light response were the most abundant in PbDof genes.Through SSR site prediction,we analyzed that the evolution level of Dof genes is low.Additionally,we assessed the expression profiles of eight PbDof genes under high temperature,drought,and light stress using qRT-PCR.In particular,PbDof08 and PbDof16 are significantly upregulated under the three stresses.[Conclusion]Phoebe bournei is a second-class endangered and protected species unique to China,and it holds significant ecological and economic value.DNA binding one zinc finger(Dof)transcription factors are plant-specific regulators.Numerous studies have demonstrated that Dof genes are involved in plant growth,development and responses to abiotic stress.Through phylogenetic analysis,it was found that PbDof11 exhibits high homology with AT5G60850.1 and PbDof07 shows high homology with AT3G50410.1.The results of collinearity analysis of genes indicated that 34 PbDof genes formed 24 pairs of homologous gene pairs.Under drought,high temperature,and light treatments,the expression patterns of seven PbDof genes were analyzed.It is speculated that PbDof08 and PbDof16 are the main genes involved in light response.Additionally,the mechanism of action of Dof genes when plants are under abiotic stress was also discussed.This study provides foundational information for PbDof genes and offers new insights for further research on the mechanism of Dof transcription factors responding to stress,as well as the adaptation of P.bournei to environmental changes.
郑珂晖;吕萌萌;钱佳颖;连怡然;刘小敏;曹世江;
福建农林大学计算与信息学院 福州 350002;福建农林大学林学院 福州 350002;林木逆境生理生态及分子生物学福建省高校重点实验室 福州 350002;福建农林大学生命科学学院 福州 350002;北京林业大学生物科学与技术学院 树木遗传育种国家重点实验室 北京 100083;福建农林大学林学院 福州 350002##林木逆境生理生态及分子生物学福建省高校重点实验室 福州 350002;
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