两种椴树属植物香气化合物比较分析及加工过程中香气化合物的变化

[Objective]This study aims to examine the various types and attributes of aroma compounds present in the flowers of Tilia miqueliana and Tilia cordata.It seeks to identify disparities and underlying molecular mechanisms of aroma compounds between the two Tilia species,as well as to assess alterations in aroma compound content following three distinct drying procedures applied to T.miqueliana and T.cordata.[Method]It focused on examining the flowers of T.miqueliana and T.cordata under varying drying conditions at their peak flowering stages.Qualitative and quantitative analysis of compounds was conducted using the HS-SPME-GC-MS method.The orthogonal partial least squares discriminant model(OPLS-DA)was employed to identify differential metabolites between the samples.Differential metabolites were further annotated using KEGG annotation,and sensory flavor characteristics were also documented.[Result]A total of 442 compounds were identified,with terpenoids being the most diverse and heterocyclic compounds being the primary contributors to aroma.Among these,76 compounds were identified as key contributors to the distinctive aroma of Tilia,including α/β-hexon,2-isobutyl-3-methoxypyrazine,(Z)-6-nonenal,methyl benzoate,(E)-2-hexenal,1-hexanol,2-thiophenemethanethiol,p-cymene,furaneol,and(Z)-4-heptenal.The Tc samples displayed a higher concentration of volatile compounds compared to Tm,indicating a more pronounced aroma profile.[Conclusion]The current investigation lays a theoretical foundation for further revealing the molecular mechanisms underlying the production of fragrance in Tilia flowers and the exploration of insect pollination.Furthermore,it serves as the basis for the creation and use of Tilia flower tea and other related products.