荧光假单胞菌接种可通过改变叶际微生物群落促进旱柳的生长,超越施用氮肥的效果

[Objective]The enhancement of plant growth by soil fertilization and microbial inoculation involves different mechanisms,in particular by altering the phyllosphere microbiome.[Method]This study investigated the effects of nitrogen(N)fertilization,Pseudomonas fluorescens strain R124 inoculation and their combination on Salix matsudana cuttings of different ages.We focused on assessing changes in the phyllosphere microbial community,plant growth parameters and soil nutrient dynamics.[Result]The results showed that P.fluorescens inoculation was more effective than N fertilization alone,promoting significant increases in biomass,plant tissue nutrient uptake,soil nutrient improvement and root development.In particular,inoculation significantly shifted the beta-diversity of the phyllosphere microbial community,with K-strategy fungi significantly enhancing plant N fixation and consequently growth.Responses varied between cuttings from young and middle-aged forests,with those from middle-aged forests showing more robust growth.This variation was linked to differential effects on phyllosphere fungi,particularly the relative abundance of Myriangiales in young and Capnodiales in middle-aged cuttings,which may enhance phyllosphere stability and plant health.[Conclusion]These results highlight the superior efficacy of microbial inoculation over N fertilization in promoting plant growth through modulation of phyllosphere microbial communities,and provide insights for sustainable management of willow plantations at different developmental stages.