光质对沙氏鹿茸草组培苗生长生理和玻璃化转变的影响
The effect of light quality on the growth physiology and vitrification transition of tissue cultured seedlings of Monochasma salvatieri Franch.ex Maxim
[目的]沙氏鹿茸草在组培过程中存在玻璃化、生长不良等现象，试验旨在探究光质在沙氏鹿茸草组织培养中是否有壮苗和缓解玻璃化作用，以期为鹿茸草的规模化生产提供理论基础。[方法]本研究以2个沙氏鹿茸草优良无性系为材料，比较纯蓝光、纯红光、红蓝1:1、红蓝1:3以及红蓝3:1和对照荧光间组培苗生长、生理生化的差异。[结果](1)纯红光显著促进JXFY1的苗高和干重，与对照相比，纯红光处理下，分别增加了 16%和14%，纯红光、红蓝 1:1、红蓝1:3 显著提高了POD活性和SOD活性，与对照相比，分别提高了 99.8%、74.5%、47.3%和 89.5%、157.3%和 95.6%;纯红光和纯蓝光显著提高了可溶性糖和可溶性蛋白的含量，与对照相比，分别提高了 102.6%、33.7%和42.4%、41.4%，并且纯红光还显著提高了其类胡萝卜素的含量。(2)纯蓝光、纯红光、红蓝1:1、红蓝1:3 和红蓝3:1处理均显著降低了ZJLS13 的玻璃化率，与对照相比，玻璃化率分别降低了 64%、87.9%、45.5%、69.7%和 60.6%;纯红光处理下的POD活性和SOD活性均显著高于对照，与对照相比，分别提高了 20.9%和 46.7%;纯蓝光和纯红光下的可溶性糖含量和可溶性蛋白含量均显著高于对照，与对照相比，分别提高了 31.7%、26.3%和62.5%、66.3%;对照下的叶绿素a、叶绿素b以及叶绿素(a+b)均显著低于纯红光处理。(3)利用隶属函数法进行综合评价，纯红光处理在JXFY1 和ZJLS13 组培苗中综合评分最高。[结论]光质对JXFY1和ZJLS13 组培苗的生长和生理均有显著影响，应选择纯红光促进JXFY1无性系组培苗生长并降低ZJLS1 3组培苗的玻璃化率。
[Objective]There are phenomena such as vitrification and poor growth in the tissue culture process of Monochasma salvatieri.The experiment aims to explore whether light quality has a strong seedling and alleviates vitrification effect in Monochasma salvatieri tissue culture,in order to provide a theoretical basis for the large-scale production of Monochasma salvatieri.[Method]This study used two superior clones of Monochasma salvatieri as materials to compare the differences in tissue culture seedling growth,physiological and biochemical characteristics between pure blue light,pure red light,red blue 1:1,red blue 1:3,and red blue 3:1 with control fluorescence.[Result](1)Pure red light significantly promoted the seedling height and dry weight of JXFY1.Compared with the control,pure red light treatment increased by 16%and 14%,respectively.Pure red light,red blue 1:1,and red blue 1:3 significantly increased POD and SOD activities.Compared with the control,pure red light treatment increased by 99.8%,74.5%,47.3%,89.5%,157.3%,and 95.6%,respectively;Pure red light and pure blue light significantly increased the content of soluble sugars and soluble proteins,with increases of 102.6%,33.7%,42.4%,and 41.4%compared to the control,respectively.Pure red light also significantly increased the content of carotenoids.(2)Pure blue light,pure red light,red blue 1:1,red blue 1:3,and red blue 3:1 treatments all significantly reduced the glass transition rate of ZJLS13.Compared with the control,the glass transition rate decreased by 64%,87.9%,45.5%,69.7%,and 60.6%,respectively;The POD activity and SOD activity under pure red light treatment were significantly higher than the control,with an increase of 20.9%and 46.7%,respectively,compared to the control;The soluble sugar content and soluble protein content under pure blue and pure red light were significantly higher than the control,with an increase of 31.7%,26.3%,and 62.5%,66.3%,respectively,compared to the control;The chlorophyll a,chlorophyll b,and chlorophyll(a+b)under control were significantly lower than those under pure red light treatment.(3)Using the membership function method for comprehensive evaluation,pure red light treatment had the highest comprehensive score in JXFY1 and ZJLS13 tissue cultured seedlings.[Conclusion]Light quality has a significant impact on the growth and physiology of JXFY1 and ZJLS13 tissue cultured seedlings.Pure red light should be chosen to promote the growth of JXFY1 asexual tissue cultured seedlings and reduce the vitrification rate of ZJLS13 tissue cultured seedlings.
罗桂生;刘素贞;李峰卿;邹玉玲;罗家萱;曾满生;黄维;
中国林业科学研究院亚热带林业实验中心 江西分宜 336600;中国林业科学研究院亚热带林业实验中心 江西分宜 336600;中国林业科学研究院亚热带林业实验中心 江西分宜 336600;中国林业科学研究院亚热带林业实验中心 江西分宜 336600;中国林业科学研究院亚热带林业实验中心 江西分宜 336600;中国林业科学研究院亚热带林业实验中心 江西分宜 336600;中国林业科学研究院亚热带林业实验中心 江西分宜 336600;
Monochasma salvatieri Franch ex Maxim LED light quality growth physiology vitrification
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