桉叶油素型和柠檬醛型樟树精油抗氧化研究
Antioxidant Activity of Eucalyptus-type and Citral-typeCamphor Essential Oils
[目的]探究桉叶油素型和柠檬醛型樟树精油及其纯露的抗氧化性。[方法]采用水蒸气蒸馏法提取桉叶油素型和柠檬醛型樟树精油，气相色谱质谱联用分析仪(GC-MS)分析其化学成分及相对含量。[结果]桉叶油素型樟树精油中相对含量0.1%以上的化学成分有 15种，其中 1，8-桉叶油素相对含量最高为57.28%，精油浓度为128 g·L-1时，对1，1-二苯基-2-三硝基苯肼(DPPH)清除率(82.60%)高于阳性对照(77.85%)，对DPPH和2，2-联氮-二(3-乙基-苯并噻唑-6-磺酸)二铵盐(ABTS)清除能力最强的半抑制浓度(IC50)分别为 14.25、31.65 g·L-1.柠檬醛型樟树精油中相对含量 0.1%以上的化学成分有30种，柠檬醛(橙花醛和香叶醛)相对含量最高为42.32%，对DPPH和ABTS清除能力最强的IC50分别为 44.45、34.46 g·L-1.精油纯露对DPPH和ABTS有一定的清除效果，且桉叶油素型樟树精油抗氧化效果(DP PH:53.24%，ABTS:4.86%)优于柠檬醛型樟树精油(DPPH:10.58%，ABTS:9.06%)。[结论]两种化学型樟树精油及其纯露均具有抗氧化性，且在一定浓度范围内高浓度精油的抗氧化性显著强于低浓度，桉叶油素型樟树精油抗氧化性强于柠檬醛型樟树精油。研究可为樟树精油的开发利用提供理论依据。
[Objective]In order to explore the antioxidant properties of eucalyptus and citral camphoressential oils and hydrosols.[Method]Eucalyptus and citral camphor essential oils were extracted by steam distillation, and their chemical composition and relative content were analyzed by gas chromatography-mass spectrometry analyzer (GC-MS).[Result]There were 15 major compounds with a relative content of more than 0.1% in eucalyptus-type camphor essential oil, among which Eucalyptol had the highest content of 57.28%. At the essential oil concentration of 128 g•L-1, the scavenging rate of essential oil to 1,1-diphenyl-2-picrylhydrazyl (DPPH) was 82.60%, which was higher than that of the positive control(77.85%). The half maximal inhibitory concentration (IC50) with the strongest scavenging capacity for DPPH and 2,2-Azinobis-(3-ethylbenzthiazoline-6-sulphonate) (ABTS) were 14.25 and 31.65 g•L-1 respectively. There were 30 major compounds with a relative content more than 0.1% in citral-type camphor essential oil, among which citral (neral and geranial) had the highest content of 42.32%. The IC50 with the strongest scavenging capacity for DPPH and ABTS were 44.45 and 34.46 g•L-1 respectively. The hydrosols of essential oil had a certain scavenging effect on DPPH and ABTS, and the antioxidant effect of eucalyptustype camphor (DPPH: 53.24%, ABTS: 4.86%) was better than citral-type camphor(DPPH: 10.58%, ABTS:9.06%).[Conclusion]Both chemotypes of camphor essential oils and hrdrosols possessed antioxidant properties, and in a certain concentration range, the antioxidant properties of the essential oils were significantly stronger at higher concentrations than the lower concentrations. Essential oil of eucalyptus-type camphor had stronger antioxidant activity than citral-type camphor. The research provide a theoretical basis for the development and utilization of camphor essential oil.
杨伟;张北红;另青艳;钟庆;余佳敏;肖祖飞;金志农;刘苑秋;
南昌工程学院水土保持学院/江西省樟树繁育与开发利用工程研究中心,江西 南昌 330099##华中农业大学园艺林学学院,湖北 武汉 430000;南昌工程学院水土保持学院/江西省樟树繁育与开发利用工程研究中心,江西 南昌 330099;南昌工程学院水土保持学院/江西省樟树繁育与开发利用工程研究中心,江西 南昌 330099##江西农业大学林学院,江西 南昌 330099;吉安市园林绿化中心,江西 吉安 343000;南昌工程学院水土保持学院/江西省樟树繁育与开发利用工程研究中心,江西 南昌 330099;南昌工程学院水土保持学院/江西省樟树繁育与开发利用工程研究中心,江西 南昌 330099;南昌工程学院水土保持学院/江西省樟树繁育与开发利用工程研究中心,江西 南昌 330099;江西农业大学林学院,江西 南昌 330099;
Cinnamomum camphora essential oil citral eucalyptus antioxidant
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