不同生境沙氏鹿茸草根际土壤细菌群落结构和多样性分析

[Objective]This study is devoted to the relationship between bacterial community structure and diversity and soil physical and chemical properties in the rhizosphere soil of wild and artificially cultivated Monochasma savatieri Franch ex Maxim.in Jiangxi Province,the aim is to build a healty rhizosphere microenvironment and provide theoretical basis for further exploring the bionic cultivation mode of M.savatieri.[Method]The rhizosphere soil bacteria in two habitats,including the natural forest such as Camellia oleifera,Gardenia jasminoides,Pinus massoniana and artificial cultivation were selected as the research objects.The community structure characteristics in different habitats of soil bacteria were compared and analyzed by using field investigation,Illumina Miseq sequencing,and RDA analysis.[Result]The results indicated that there were significant differences in the soil physio-chemical properties of the rhizosphere soil of M.savatieri in two habitats,and the alpha diversity of artificial bionic cultivation was highest,but did not reach a significant level among different stand types.In two habitats,Proteobacteria,Acidobacteriota,Chloroflexi and Actinobacteriota were the dominant bacteria phyla;At the genus level,the common dominant genera included Burkholderia-Caballeronia-Paraburkholderia,Bradyrhizobium,Candidatus_Solibacter,Occallatibacter,Bryobacter and uncultured_bacterium.The structure of the bacterial communities between wild and artificial bionic cultivation was significant differences,with the highest abundance of Proteobacteria in artificial bionic cultivated habitats.The unique dominant genera in wild habitats included Acidothermus,FCPS473,Conexibacter,Acidibacter,uncultured_Acidobacteria_bacterium,uncultured_forest_soil_bacterium significantly decreased in artificial bionic cultivation habitat,while compared to wild habitat,Sphingomonas,Granulicella,Haliangium and Mucilaginibacter,which only dominated in artificial bionic cultivated habitat,increased significantly.Redundancy analysis(RDA)suggested that total nitrogen,available potassium,alkali-hydrolyzable nitrogen,and organic matter were the dominant influencing factors on the composition of soil dominant bacterial communities.[Conclusion]Under different habitats,there were significant differences in the physicochemical properties of the rhizosphere soil of M.savatieri,as well as significant differences in the structure of bacterial communities.The similarity between the rhizosphere bacterial communities in the rhizosphere of M.savatieri under wild habitat conditions was relatively high.The main environmental factors affecting bacterial community structure were soil total nitrogen,available potassium,alkaline nitrogen,and organic matter.This study provides reference basis for the resources and utilization of rhizosphere micoorganisms of M.savatieri..