垂珠花叶饮品有效成分时空分布规律及其资源价值评价

This study focused on the growth and development of Styrax dasyanthus leaves,the accumulation of metabolites and gene expression and metabolite changes under different leaf colour conditions,and the trial production and evaluation of S.dasyanthus leaf tea of different processes,aiming to provide scientific basis and technical support for the efficient use of resources and the development of health care beverages of S.dasyanthus leaves. 1.There were differences in water content,chlorophyll content,organic matter accumulation rate and flavonoid metabolite accumulation in different parts and periods of leaves.Overall,the upper leaves of S.dasyanthus had low water content and low chlorophyll content,and the middle leaves had the most stable accumulation of flavonoid secondary metabolites,while the middle and lower leaves had high chlorophyll content and rich accumulation of organic matter.Collection strategy:It is recommended to collect upper leaves from spring to early summer;middle leaves from mid-summer;and lower leaves from late summer to autumn to obtain higher levels of organic matter and metabolites. 2.The richness and multiple medicinal values of the metabolic constituents of the leaves of S.dasyanthus include flavonoids,phenolic acids,amino acids and their derivatives,lignans,coumarins,lipids,terpenoids,alkaloids,organic acids,nucleotides and their derivatives,tannins,and quinones.These metabolic components provide the biological basis for the health benefits of the leaves of S.dasyanthus,with antioxidant,anticancer,anti-inflammatory and many other effects.Flavonoids,alkaloids,phenolic acids and terpene metabolites are the main active ingredients in the leaves. 3.The anthocyanin content in the leaves of the purple-leafed S.dasyanthus is significantly higher than that of the green leaves,which has its unique medicinal value.The main reason is that the external conditions of light and water affect the pigment accumulation and metabolite differences in the leaves of Pittosporum by controlling gene expression.The two key differential modules of purple and green leaves of S.dasyanthus show synergistic effects in the development of plant leaf colour by regulating biological processes such as cellular material metabolism,cellular processes and stress responses.transcription factor families such as MYB,bHLH and AP2/ERF play key roles in the regulation of flavonoid biosynthesis and other key metabolic pathways.This opens up the possibility of selecting and breeding pitcher flower varieties with specific medicinal values by means of environmental regulation. 4.It is feasible to make various kinds of beverages from S.dasyanthus leaves.The green tea and black tea made from S.dasyanthus leaves have special aroma,among which the black tea processed with S.dasyanthus leaves has better performance in terms of taste,aroma and appearance.In addition,the highly twisted and long-term fermented tea is not only in a better form,but also easier to transport and preserve,as well as to drink and promote. In conclusion,the present study provides a solid scientific foundation for the rational harvesting,resource utilisation and health drink development of S.dasyanthus leaves,reveals the rich metabolites and biological functions of S.dasyanthus leaves,and provides a new direction for the cultivation management,resource utilisation and medicinal value development of S.dasyanthus.