Genome-wide identification of the bHLH transcription factor family and the regulatory roles of PbbHLH74 in response to drought stress in Phoebe bournei
Genome-wide identification of the bHLH transcription factor family and the regulatory roles of PbbHLH74 in response to drought stress in Phoebe bournei
Phoebe species constitute a large portion of subtropical forestry, which are a key player in biomass resources. Basic helix-loop-helix (bHLH) as the second largest transcription factor family plays essential roles in response to multiple stresses in plants. However, little information of bHLH family is available in Phoebe bournei. In this study, 130 PbbHLHs were identified and classified into 24 subfamilies. The bHLH domain, conserved motifs and gene structures, evolutionary patterns and protein structural features were probed. The expression of 17 PbbHLHs was differentially induced by PEG and ABA, indicating that they may play essential roles in drought stress response. PbbHLH74 was transcriptionally active and localized in the nucleus. PbbHLH74 heterologous transformation of yeast improved cellular drought tolerance. Overexpressing PbbHLH74 in Arabidopsis enhanced drought resistance by increasing the expression levels of stress-related genes. Transgenic PbbHLH74 P. bournei by hairy-rooting improved drought tolerance by enhancing root development and expression levels of genes involved in the ABA pathway and ROS scavenging. Moreover, PbbHLH74 might positively regulate the expression of PbPOD by Y1H and dual-luciferase reporter assays. Overall, these results elucidated the structure and evolution of the PbbHLH family and PbbHLH74 could be applied to molecular assisted breeding for drought tolerance in P. bournei.
Ning-Ning Fu;Li Wang;Qing-Lin Sun;Qi-Guang Wang;Yu-Ting Zhang;Xiao Han;Qi Yang;Zai-Kang Tong;Jun-Hong Zhang;
State Key Laboratory of Subtropical Silviculture,School of Forestry&Biotechnology,Zhejiang A&F University,Lin'an,Hangzhou 311300,Zhejiang,China;State Key Laboratory of Subtropical Silviculture,School of Forestry&Biotechnology,Zhejiang A&F University,Lin'an,Hangzhou 311300,Zhejiang,China;State Key Laboratory of Subtropical Silviculture,School of Forestry&Biotechnology,Zhejiang A&F University,Lin'an,Hangzhou 311300,Zhejiang,China;State Key Laboratory of Subtropical Silviculture,School of Forestry&Biotechnology,Zhejiang A&F University,Lin'an,Hangzhou 311300,Zhejiang,China;State Key Laboratory of Subtropical Silviculture,School of Forestry&Biotechnology,Zhejiang A&F University,Lin'an,Hangzhou 311300,Zhejiang,China;State Key Laboratory of Subtropical Silviculture,School of Forestry&Biotechnology,Zhejiang A&F University,Lin'an,Hangzhou 311300,Zhejiang,China;State Key Laboratory of Subtropical Silviculture,School of Forestry&Biotechnology,Zhejiang A&F University,Lin'an,Hangzhou 311300,Zhejiang,China;State Key Laboratory of Subtropical Silviculture,School of Forestry&Biotechnology,Zhejiang A&F University,Lin'an,Hangzhou 311300,Zhejiang,China;State Key Laboratory of Subtropical Silviculture,School of Forestry&Biotechnology,Zhejiang A&F University,Lin'an,Hangzhou 311300,Zhejiang,China;
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