不同种源木荷种实及苗期表型性状分析及综合评价
Analysis and comprehensive evaluation of phenotypic traits of Schima superba seeds and seedlings from different sources
[目的]研究湖南省不同种源木荷的种实及苗期表型性状多样性,为木荷种质资源收集、评价和利用提供科学依据。[方法]选择12个种源采集果实、种子、叶片,繁育 1年生苗木,测定果横径、果纵径、果柄长、叶长、叶宽、叶柄长、种子长、种子宽、地径和苗高等 10 个表型性状,利用方差分析、多重比较、相关性分析和聚类分析等方法,探究不同种源木荷的种实和苗期表型性状变异及其与环境因子的关系。[结果]木荷8个种实与叶表型性状在种源间存在广泛变异,变异系数为 8.9~25.82%,果横径、果柄长、叶宽、叶柄长存在显著性差异。(2)不同种源木荷各表型性状与地理环境之间存在不同程度的相关性,果横径、叶长、叶宽、叶柄长、种子长和种子宽与北纬、pH值存在显著负相关,苗高与东经呈显著负相关,与pH值呈极显著正相关。苗期地径变幅4.23~5.71mm,苗高变幅30.10~45.00cm,桑植县、龙山县与茶陵县、长沙县木荷苗高呈显著性差异。苗期地径生长大于平均值百分比为 1.28~16.6%,苗高生长大于平均值百分比为0.84~17.84%。(4)通过聚类分析将 12个木荷种源分为四大类型,第Ⅰ类包括金洞区、通道县、古丈县和泸溪县等4个湘西南产地,果大、叶宽、生长快;第Ⅱ类包括靖州县、南山公园、城步县和资兴市等 4 个湘东南产地,果大,叶长、叶宽,生长较快;第Ⅲ类包括茶陵县和长沙县等湘东产地,果小、生长一般;第Ⅳ类包括龙山县和桑植县等湘北产地,果小、叶短、叶窄、种子小,生长快。[结论]果横径、果柄长、叶宽、叶柄长是木荷种源地理变异主要性状。东经、北纬、pH值是影响种实表型性状的主要因素。以苗高为标准筛选出优良种源产区为桑植县、龙山县、南山公园和靖州县。
[Objective]To study the diversity of seed and seedling phenotypic traits of Schima superba from different sources in Hunan Province,and provide scientific basis for the collection,evaluation,and utilization of Schima superba germplasm resources.[Method]12 seed sources were selected to collect fruits,seeds,and leaves,and 1-year-old seedlings were bred.Ten phenotypic traits,including fruit transverse diameter,fruit longitudinal diameter,fruit stem length,leaf length,leaf width,leaf stem length,seed length,seed width,ground diameter,and seedling height,were measured.Using methods such as analysis of variance,multiple comparisons,correlation analysis,and cluster analysis,the variation of seed and seedling phenotypic traits in different seed sources of Schima superba and their relationship with environmental factors were explored.[Result]There was extensive variation in the fruit and leaf surface traits of 8 Schima superba varieties among their sources,with a coefficient of variation of 8.9-25.82%.There were significant differences in fruit diameter,stem length,leaf width,and stem length.(2)There is varying degrees of correlation between the phenotypic traits of different Schima superba species and their geographical environment.There is a significant negative correlation between fruit diameter,leaf length,leaf width,petiole length,seed length,and seed width with north latitude and pH value,a significant negative correlation between seedling height and east longitude,and an extremely significant positive correlation with pH value.The diameter variation during the seedling stage is 4.23-5.71mm,and the height variation is 30.10-45.00cm.There is a significant difference in the height of Schima superba seedlings between Sangzhi County,Longshan County,Chaling County,and Changsha County.The percentage of aboveground diameter growth exceeding the average value during the seedling stage is 1.28-16.6%,and the percentage of aboveground height growth exceeding the average value is 0.84-17.84%.(4)Through cluster analysis,12 Schima superba germplasm sources were classified into four major types.The first type includes four southwestern Hunan producing areas:Jindong District,Tongdao County,Guzhang County,and Luxi County,with large fruits,wide leaves,and fast growth;The second category includes four production areas in southeastern Hunan,including Jingzhou County,Nanshan Park,Chengbu County,and Zixing City,with large fruits,long and wide leaves,and fast growth;The third category includes Xiangdong producing areas such as Chaling County and Changsha County,with small fruits and average growth;The fourth category includes Xiangbei producing areas such as Longshan County and Sangzhi County,with small fruits,short leaves,narrow leaves,small seeds,and fast growth.[Conclusion]The lateral diameter,stem length,leaf width,and stem length of Schima superba are the main geographic variation traits.East longitude,north latitude,and pH value are the main factors affecting the phenotypic traits of seeds and fruits.Based on the standard of seedling height,excellent seed production areas were selected as Sangzhi County,Longshan County,Nanshan Park,and Jingzhou County.
唐洁;陈建军;李永进;易剑;杨艳;黎蕾;汤玉喜;
湖南省林业科学院,湖南 长沙 410004;沅江市林业科学研究所,湖南 益阳 413100;湖南省林业科学院,湖南 长沙 410004;汨罗市白水苗圃,湖南 汨罗,414400;湖南省林业科学院,湖南 长沙 410004;湖南省林业科学院,湖南 长沙 410004;湖南省林业科学院,湖南 长沙 410004;
Schima superba Seed source Phenotypic traits genetic variation
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